Microarray > Membranes for Transfer and Immobilization
 
    Membranes for Transfer and Immobilization

Descriptions:
A Variety of Membrane Chemistries for Sensitive Detection and Consistent Results in All Applications and Detection Systems
• BioTrace NT membrane—pure unsupported nitrocellulose is used for colony and plaque lifts and has low burn-through in protein transfer applications.
• BioTrace PVDF membrane—versatile membrane with broad chemical resistance, ideally suited for protein transfers.
• Biodyne membranes—intrinsically hydrophilic Nylon 6,6 membranes provide high sensitivity and low background for enhanced resolution. Ideal for nucleic acid blots and protein ELISA tests.
• FluoroTrans PVDF membranes—increased sensitivity, ideal for a wide variety of protein analysis applications including sequencing and western transfers.
• UltraBind membrane—affinity membrane is recommended for covalent protein binding.

Applications :
• Nucleic acid and protein transfer and detection:
• Northern, Southern, and Western transfers y
• Colony and plaque lifts
• Replica plating
• Dot/slot blots
• DNA fingerprinting
• Protein sequencing
• Solid phase ELISAs
• Affinity separations
• Macroarrays
• Microarrays

Transfer and Affinity Membrane Selection Guide
Product Biodyne® A Membrane Biodyne B/Plus Membrane Biodyne C Membrane
Description Amphoteric Nylon 6,6 Positively-charged Nylon 6,6 Negatively-charged Nylon 6,6
Works best for Colony/Plaque Lifts, DNA and RNA Transfers DNA and RNA Transfers, Multiple Reprobings Reverse Dot Blots
Also suited for Gene Probe Assays, DNA Fingerprinting, Nucleic Acid Dot/Slot Blots, Replica Plating, ELISAs DNA Fingerprinting, Nucleic Acid Dot/Slot Blots, Colony/Plaque Lifts (Biodyne B membrane), Replica Plating (Biodyne B membrane) Protein Immobilization, Affinity Purification, ELISAs
Advantages
• High sensitivity
• Low background
• Net charge can be controlled by changing pH
• Ability to strip and reprobe
• Positive charge over broad pH range
• Highest sensitivity for nucleic acid applications (Biodyne B membrane)
• Ability to strip and reprobe
• Negative charge over broad pH range
• Surface carboxyl groups can be derivatized
• Ability to strip and reprobe
Binding Interaction Hydrophobic & Electrostatic Hydrophobic & Electrostatic Hydrophobic & Electrostatic
Method of Immobilization UV Crosslink Baking Can be baked or UV crosslinked, although not required Derivatization
Detection Methods
Radiolabeled Probes, Enzyme-antibody Conjugates
• Chemiluminescent
• Chromogenic
Radiolabeled Probes, Enzyme-antibody Conjugates
• Chemiluminescent
• Chemifluorescent (Biodyne Plus Membrane)
Radiolabeled Probes, Enzyme-antibody Conjugates
• Chromogenic

Product BioTrace™ NT Membrane BioTrace PVDF Membrane FluoroTrans® Membrane UltraBind™ Membrane
Description 100% Pure Nitrocellulose Polyvinylidene Fluoride Polyvinylidene Fluoride Modified Polyethersulfone
Works best for Colony/Plaque Lifts Protein Transfers Western Transfers (FluoroTrans W) N-terminal Protein Sequencing (FluoroTrans PVDF) Solid-phase ELISAs
Also suited for Nucleic Acid and Protein Transfers, Protein Dot/Slot Blots Protein Dot/Slot Blots   Affinity Chromatography, Hybridoma Screening
Advantages
• Excellent strength
• No support fabric
• No detergents added
• 100% pure nitrocellulose
• Chemical resistance
• No discoloration
• Nonflammable
• High strength
• Strong protein binding
• Sensitive detection
• Very low burn-through
• Good chemical compatibility
• Covalent binding
• No preactivation required
• High protein-binding capacity
Binding Interaction Hydrophobic & Electrostatic Hydrophobic Hydrophobic Covalent
Method of Immobilization UV Crosslink Baking (Vacuum Oven)     Direct Spotting Perfusion
Detection Methods
Radiolabeled Probes, Direct Stain, Fluorescence, Enzyme-antibody Conjugates
• Chemi-luminescent
• Chromogenic
Direct Stain, Enzyme-antibody Conjugates
• Chemi-luminescent
• Chromogenic
Direct Stain with Coomaisse blue, Amido black, Ponceau S, and colloidal gold(FluoroTrans W membrane). Enzyme-antibody Conjugates
• Chemiluminescent
• Chromogenic
Radiolabeled Probes, Enzyme-antibody Conjugates
• Chromogenic

Pall BioTrace NT Membrane
Cat no. Descriptions Quantity
66487 82 mm discs 50/pkg
66595 85 mm discs 50/pkg
66518 132 mm discs 50/pkg
66488 132 mm discs 50/pkg
66593 7 x 8.5 cm sheets 10/pkg
66489 20 x 20 cm sheets 10/pkg
66485 30 cm x 3 m roll 1/pkg

Pall BioTrace PVDF Membrane
Cat no. Descriptions Quantity
66594 7 x 8.5 cm sheets 10/pkg
66542 20 x 20 cm sheets 10/pkg
66543 30 cm x 3 m roll 1/pkg
66547 20 cm x 1 m roll 1/pkg

Pall Biodyne A Membrane
Cat no. Descriptions Quantity
60113 0.2 µm,30 cm x 3 m roll 1/pkg
60102 0.45 µm,82 mm discs 50/pkg
60103 0.45 µm,85 mm discs 50/pkg
60104 0.45 µm,132 mm discs 50/pkg
60105 0.45 µm,137 mm discs 50/pkg
60101 0.45 µm,7 x 8.5 cm sheets 10/pkg
60100 0.45 µm,20 x 20 cm sheets 10/pkg
60106 0.45 µm,30 cm x 3 m roll 1/pkg
60120 0.45 µm,20 cm x 3 m roll 1/pkg
60108 1.2 µm, 30 cm x 3 m roll 1/pkg

Pall Biodyne B Membrane
Cat no. Descriptions Quantity
60202 0.45 µm,82 mm discs 50/pkg
60203 0.45 µm,82 mm discs 50/pkg
60204 0.45 µm,132 mm discs 50/pkg
60205 0.45 µm,137 mm discs 50/pkg
60201 0.45 µm,7 x 8.5 cm sheets 10/pkg
60200 0.45 µm,20 x 20 cm sheets 10/pkg
60207 0.45 µm,30 cm x 3 m roll 1/pkg
60209 0.45 µm,20 cm x 1 m roll 1/pkg
60208 0.45 µm,20 cm x 3 m roll 1/pkg

Pall Biodyne C Membrane
Cat no. Descriptions Quantity
60316 0.45 µm,82 mm discs 50/pkg
60317 0.45 µm,82 mm discs 50/pkg
60318 0.45 µm,132 mm discs 50/pkg
60319 0.45 µm,137 mm discs 50/pkg
60315 0.45 µm,7 x 8.5 cm sheets 10/pkg
60314 0.45 µm,20 x 20 cm sheets 10/pkg
60320 0.45 µm,30 cm x 3 m roll 1/pkg

Pall Biodyne Plus Membrane
Cat no. Descriptions Quantity
60402 0.45 µm,82 mm discs 50/pkg
60403 0.45 µm,82 mm discs 50/pkg
60404 0.45 µm,132 mm discs 50/pkg
60405 0.45 µm,137 mm discs 50/pkg
60401 0.45 µm,7 x 8.5 cm sheets 10/pkg
60400 0.45 µm,20 x 20 cm sheets 10/pkg
60406 0.45 µm,30 cm x 3 m roll 1/pkg

Pall FluoroTrans W Membrane
Cat no. Descriptions Quantity
BSP0158 0.2 µm,7 x 9 cm sheets 10/pkg
BSP0517 0.2 µm,10 x 15 cm sheets 10/pkg
BSP0159 0.2 µm,20 x 20 cm sheets 10/pkg
BSP0161 0.2 µm,3.3 m roll 1/pkg

Pall FluoroTrans Membrane
Cat no. Descriptions Quantity
PVM020C-160 0.2 µm,7 X 8.5 cm sheets 10/pkg
PVM020C-195 0.2 µm,8.5 X 9 cm sheets 20/pkg
PVM020C1015 0.2 µm,10 x 15 cm sheets 10/pkg
PVM020C-196 0.2 µm,13 x 14 cm sheets 10/pkg
PVM020C2020 0.2 µm,20 x 20 cm sheets 10/pkg
PVM020C-099 0.2 µm,3.3 mn roll 1/pkg

Pall UltraBind Membrane
Cat no. Descriptions Quantity
66544 0.45 µm,20 x 20 cm sheets 10/pkg
66546 0.45 µm,30 cm x 3 m roll 1/pkg

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