Custom Services > Oligo Synthesis
 
Oligo Synthesis Service
• Highest Purity at Lowest Price
• Specializing in HAP* (High Affinity Purification) and PAGE Purification
• Fast Services: Synthesis Completed within 36 Hours (for HAP* or PAGE)
• All modified base oligos are purified by HPLC (WAVE system)
• Highest QC and Quality Guaranteed

High Affinity Purification (HAP) is a patented, novel purification method for custom oligos developed by Bio Basic Inc. DMT-ON-Oligo in the crude oligo mixture is first selectively absorbed on a high affinity resin in HAP column while incomplete oligos pass through. Final oligo is produced by removing the protection group of DMT under mild acidic conditions. HAP method provides two major advantages, high purity superior to De-Salted method (>98%), and low cost comparable to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis. At present, the most economic method to produce oligos is the De-Salted method, which however, yields products poor in purity. For example, the purity of 20-mer, 40-mer and 60-mer is approximately 68%, 45% and 30% respectively. This is calculated based on the 4 steps in DNA synthesis: De-DMT, Coupling, Oxidation and Capping. The average yield of each cycle is about 98%. The purity of 20-mer is therefore (0.98)20-1 = 68%; of 40-mer = (0.98)40-1 = 45%; and of 60-mer = (0.98)60-1 = 30%. Most laboratories use De-Salted oligos despite of their inferior purity because higher quality oligos produced by alternative methods such as PAGE, HPLC, and OPC are too costly. HAP presents the perfect alternative for high purity oligos at lowest prices. In fact, price is even lower than those of De-Salted method in some cases.
 
How to Order
Download and Email: You may download the Oligo Synthesis Order Form and submit your order via e-mail to info@mdgsb.com or ict@mdgsb.com

Note: Please indicate scales, purification method, 5' end or 3' end modification. To guarantee quality, we do not carry De-salted oligos. HAP is a superior purification scale as compared to Desalt.

 Regular Oligos synthesis

 HAP Purification
Item No. Synthesis Scale Available Lengths Yield
(5-9base)		 Yield
(10-60base)
HAP-100 100 nmole 5-60base 2 ODs 5 ODs
HAP-200 200 nmole 5-60base 5 ODs 10 ODs
HAP-400 400 nmole 5-60base 10 ODs 20 ODs
HAP-1000 1 µmole 5-60base 20 ODs 40 ODs

 PAGE Purification (Mass QC)
Item No. Synthesis Scale Available Lengths Yield
(10-60base)		 Yield
(61-90base)		 Yield
(91-110base)
PAGE-100 100 nmole 10-60base 2 ODs N/A N/A
PAGE-200 200 nmole 10-90base 5 ODs 2 ODs N/A
PAGE-400 400 nmole 10-110base 10 ODs 5 ODs 2 ODs
PAGE-1000 1 µmole 10-110base 20 ODs 10 ODs 5 ODs

 HPLC Purification (Mass QC)
Item No. Synthesis Scale Available Lengths Yield
(5-9base)		 Yield
(10-60base)
HPLC-100 100 nmole 5-60base 1 OD 2 ODs
HPLC-200 200 nmole 5-60base 2 ODs 5 ODs
HPLC-400 400 nmole 5-60base 5 ODs 10 ODs
HPLC-1000 1 µmole 5-60base 10 ODs 20 ODs

 dual Purification(PAGE & HPLC) (Mass QC)
Item No. Synthesis Scale Available Lengths Yield
(10-60base)		 Yield
(61-90base)
DHPLC-100 100 nmole 10-60base 1 OD N/A
DHPLC-200 200 nmole 10-90base 2 ODs 1 OD
DHPLC-400 400 nmole 10-90base 5 ODs 2 ODs
DHPLC-1000 1 µmole 10-90base 10 ODs 5 ODs

 dI
DeoxyInosine Synthesis Scale Purification Method
DI-HAP-100 100 nmole HAP
DI-HAP-200 200 nmole HAP
DI-HAP-400 400 nmole HAP
DI-HAP-1000 1 µmole HAP
DI-PAGE-100 (mass QC) 100 nmole PAGE
DI-PAGE-200 (mass QC) 200 nmole PAGE
DI-PAGE-400 (mass QC) 400 nmole PAGE
DI-PAGE-1000 (mass QC) 1 µmole PAGE
DI-HPLC-100 (mass QC) 100 nmole HPLC
DI-HPLC-200 (mass QC) 200 nmole HPLC
DI-HPLC-400 (mass QC) 400 nmole HPLC
DI-HPLC-1000 (mass QC) 1 µmole HPLC

 dU
DeoxyInosine Synthesis Scale Purification Method
DU-HAP-100 100 nmole HAP
DU-HAP-200 200 nmole HAP
DU-HAP-400 400 nmole HAP
DU-HAP-1000 1 µmole HAP
DU-PAGE-100 (mass QC) 100 nmole PAGE
DU-PAGE-200 (mass QC) 200 nmole PAGE
DU-PAGE-400 (mass QC) 400 nmole PAGE
DU-PAGE-1000 (mass QC) 1 µmole PAGE
DU-HPLC-100 (mass QC) 100 nmole HPLC
DU-HPLC-200 (mass QC) 200 nmole HPLC
DU-HPLC-400 (mass QC) 400 nmole HPLC
DU-HPLC-1000 (mass QC) 1 µmole HPLC

 Phosphorothioate Bond (Mass QC)
Item No. Synthesis Scale
PHOS-100 100 nmole
PHOS-200 200 nmole
PHOS-400 400 nmole
PHOS-1000 1 µmole

 Phosphorylation
5' Phosphorylation Synthesis Scale Purification Method Yield(10-60base)
PH5-100 100 nmole HPLC 2 ODs
PH5-200 200 nmole HPLC 5 ODs
PH5-400 400 nmole HPLC 10 ODs
PH5-1000 1 µmole HPLC 20 ODs

3' Phosphorylation Synthesis Scale Purification Method Yield(10-60base)
PH3-100 100 nmole HPLC 2 ODs
PH3-200 200 nmole HPLC 5 ODs
PH3-400 400 nmole HPLC 10 ODs
PH3-1000 1 µmole HPLC 20 ODs

 Attachment Chemistry & Linkers
5'NH2 C6(mass QC) Synthesis Scale Purification Method Yield(10-60base)
NH65-100 100 nmole HPLC 2 ODs
NH65-200 200 nmole HPLC 5 ODs
NH65-400 400 nmole HPLC 10 ODs
NH65-1000 1 µmole HPLC 20 ODs

3'NH2 C6 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
NH63-100 100 nmole HPLC 2 ODs
NH63-200 200 nmole HPLC 5 ODs
NH63-400 400 nmole HPLC 10 ODs
NH63-1000 1 µmole HPLC 20 ODs

5'NH2 C12 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
NH125-100 100 nmole HPLC 2 ODs
NH125-200 200 nmole HPLC 5 ODs
NH125-400 400 nmole HPLC 10 ODs
NH125-1000 1 µmole HPLC 20 ODs

5'Biotin(mass QC) Synthesis Scale Purification Method Yield(10-60base)
BT5-100 100 nmole HPLC 2 ODs
BT5-200 200 nmole HPLC 5 ODs
BT5-400 400 nmole HPLC 10 ODs
BT5-1000 1 µmole HPLC 20 ODs

3'Biotin(mass QC) Synthesis Scale Purification Method Yield(10-60base)
BT3-100 100 nmole HPLC 2 ODs
BT3-200 200 nmole HPLC 5 ODs
BT3-400 400 nmole HPLC 10 ODs
BT3-1000 1 µmole HPLC 20 ODs

5' or Int Biotin dT (mass QC) Synthesis Scale Purification Method Yield(10-60base)
BTT-100 100 nmole HPLC 2 ODs
BTT-200 200 nmole HPLC 5 ODs
BTT-400 400 nmole HPLC 10 ODs
BTT-1000 1 µmole HPLC 20 ODs

5'Digoxigenin NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
DIG5-100 100 nmole HPLC 2 ODs
DIG5-200 200 nmole HPLC 5 ODs
DIG5-400 400 nmole HPLC 10 ODs
DIG5-1000 1 µmole HPLC 20 ODs

5' Thiol Modifier C6 S-S (mass QC) Synthesis Scale Purification Method Yield(10-60base)
SH5SS-100 100 nmole HPLC 2 ODs
SH5SS-200 200 nmole HPLC 5 ODs
SH5SS-400 400 nmole HPLC 10 ODs
SH5SS-1000 1 µmole HPLC 20 ODs

5' Thiol Modifier C6 SH (mass QC) Synthesis Scale Purification Method Yield(10-60base)
SH5SH-100 100 nmole HPLC 2 ODs
SH5SH-200 200 nmole HPLC 5 ODs
SH5SH-400 400 nmole HPLC 10 ODs
SH5SH-1000 1 µmole HPLC 20 ODs

3' Thiol Modifier C3 S-S (mass QC) Synthesis Scale Purification Method Yield(10-60base)
SH3SS-100 100 nmole HPLC 2 ODs
SH3SS-200 200 nmole HPLC 5 ODs
SH3SS-400 400 nmole HPLC 10 ODs
SH3SS-1000 1 µmole HPLC 20 ODs

3' Thiol Modifier C3 SH (mass QC) Synthesis Scale Purification Method Yield(10-60base)
SH3SH-100 100 nmole HPLC 2 ODs
SH3SH-200 200 nmole HPLC 5 ODs
SH3SH-400 400 nmole HPLC 10 ODs
SH3SS-1000 1 µmole HPLC 20 ODs

 Modified bases
3' Inverted dT Synthesis Scale Purification Method Yield(10-60base)
INV-100 100 nmole HPLC 2 ODs
INV-200 200 nmole HPLC 5 ODs
INV-400 400 nmole HPLC 10 ODs
INV-1000 1 µmole HPLC 20 ODs

5-Methyl dC Synthesis Scale Purification Method Yield(10-60base)
METH-100 100 nmole HPLC 2 ODs
METH-200 200 nmole HPLC 5 ODs
METH-400 400 nmole HPLC 10 ODs
METH-1000 1 µmole HPLC 20 ODs

Locked Nucleic Acids (LNA) Synthesis Scale Purification Method Yield(10-60base)
LNA-100 100 nmole HPLC 2 ODs
LNA-200 200 nmole HPLC 5 ODs
LNA-400 400 nmole HPLC 10 ODs
LNA-1000 1 µmole HPLC 20 ODs

3' Dideoxy-C(ddC) Synthesis Scale Purification Method Yield(10-60base)
DDC-100 100 nmole HPLC 2 ODs
DDC-200 200 nmole HPLC 5 ODs
DDC-400 400 nmole HPLC 10 ODs
DDC-1000 1 µmole HPLC 20 ODs

 Spacers
C3 Spacer Synthesis Scale Purification Method Yield(10-60base)
C3-100 100 nmole HPLC 2 ODs
C3-200 200 nmole HPLC 5 ODs
C3-400 400 nmole HPLC 10 ODs
C3-1000 1 µmole HPLC 20 ODs

5' or Int Spacer 9 Synthesis Scale Purification Method Yield(10-60base)
SPACER9-100 100 nmole HPLC 2 ODs
SPACER9-200 200 nmole HPLC 5 ODs
SPACER9-400 400 nmole HPLC 10 ODs
SPACER9-1000 1 µmole HPLC 20 ODs

5' or Int Spacer 18 Synthesis Scale Purification Method Yield(10-60base)
SPACER18-100 100 nmole HPLC 2 ODs
SPACER18-200 200 nmole HPLC 5 ODs
SPACER18-400 400 nmole HPLC 10 ODs
SPACER18-1000 1 µmole HPLC 20 ODs

5' or Int d Spacer Synthesis Scale Purification Method Yield(10-60base)
DSPACER-100 100 nmole HPLC 2 ODs
DSPACER-200 200 nmole HPLC 5 ODs
DSPACER-400 400 nmole HPLC 10 ODs
DSPACER-1000 1 µmole HPLC 20 ODs

 Fluorophores
5'or 3' 6-FAM(mass QC) Synthesis Scale Purification Method Yield(10-60base)
FAM-100 100 nmole HPLC 2 ODs
FAM-200 200 nmole HPLC 5 ODs
FAM-400 400 nmole HPLC 10 ODs
FAM-1000 1 µmole HPLC 20 ODs

5' TET(mass QC) Synthesis Scale Purification Method Yield(10-60base)
TET-100 100 nmole HPLC 2 ODs
TET-200 200 nmole HPLC 5 ODs
TET-400 400 nmole HPLC 10 ODs
TET-1000 1 µmole HPLC 20 ODs

5' HEX(mass QC) Synthesis Scale Purification Method Yield(10-60base)
HEX-100 100 nmole HPLC 2 ODs
HEX-200 200 nmole HPLC 5 ODs
HEX-400 400 nmole HPLC 10 ODs
HEX-1000 1 µmole HPLC 20 ODs

5' TAMRA NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
TAMRA5-100 100 nmole HPLC 2 ODs
TAMRA5-200 200 nmole HPLC 5 ODs
TAMRA5-400 400 nmole HPLC 10 ODs
TAMRA5-1000 1 µmole HPLC 20 ODs

3' TAMRA NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
TAMRA3-100 100 nmole HPLC 2 ODs
TAMRA3-200 200 nmole HPLC 5 ODs
TAMRA3-400 400 nmole HPLC 10 ODs
TAMRA3-1000 1 µmole HPLC 20 ODs

5' ROX NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
ROX5-100 100 nmole HPLC 2 ODs
ROX5-200 200 nmole HPLC 5 ODs
ROX5-400 400 nmole HPLC 10 ODs
ROX5-1000 1 µmole HPLC 20 ODs

3' ROX NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
ROX3-100 100 nmole HPLC 2 ODs
ROX3-200 200 nmole HPLC 5 ODs
ROX3-400 400 nmole HPLC 10 ODs
ROX3-1000 1 µmole HPLC 20 ODs

5' JOE NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
JOE5-100 100 nmole HPLC 2 ODs
JOE5-200 200 nmole HPLC 5 ODs
JOE5-400 400 nmole HPLC 10 ODs
JOE5-1000 1 µmole HPLC 20 ODs

3' JOE NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
JOE3-100 100 nmole HPLC 2 ODs
JOE3-200 200 nmole HPLC 5 ODs
JOE3-400 400 nmole HPLC 10 ODs
JOE-1000 1 µmole HPLC 20 ODs

5'CY3 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY5-100 100 nmole HPLC 2 ODs
CY5-200 200 nmole HPLC 5 ODs
CY5-400 400 nmole HPLC 10 ODs
CY5-1000 1 µmole HPLC 20 ODs

3' or Int CY3(mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY3-100 100 nmole HPLC 2 ODs
CY3-200 200 nmole HPLC 5 ODs
CY3-400 400 nmole HPLC 10 ODs
CY3-1000 1 µmole HPLC 20 ODs

5'CY5 NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY55-100 100 nmole HPLC 2 ODs
CY55-200 200 nmole HPLC 5 ODs

3'CY5 NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY35-100 100 nmole HPLC 2 ODs
CY35-200 200 nmole HPLC 5 ODs

 Dual-Labeled DNA Probes
5' 6-FAM-3'TAMRA NHS Ester (mass QC) Synthesis Scale Purification Method Yield(10-60base)
FT-100 100 nmole HPLC 2 ODs
FT-200 200 nmole HPLC 5 ODs
FT-400 400 nmole HPLC 10 ODs
FT-1000 1 µmole HPLC 20 ODs

5' 6-FAM-3'BHQ-1 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
FB-100 100 nmole HPLC 2 ODs
FB-200 200 nmole HPLC 5 ODs

5' 6-FAM-3'Eclipse (mass QC) Synthesis Scale Purification Method Yield(10-60base)
FE-100 100 nmole HPLC 2 ODs
FE-200 200 nmole HPLC 5 ODs

5' TET-3'TAMRA NHS Ester(mass QC) Synthesis Scale Purification Method Yield(10-60base)
TT-100 100 nmole HPLC 2 ODs
TT-200 200 nmole HPLC 5 ODs

5' HEX-3'TAMRA NHS Ester(mass QC) Synthesis Scale Purification Method Yield(10-60base)
HT-100 100 nmole HPLC 2 ODs
HT-200 200 nmole HPLC 5 ODs

5' HEX-3'BHQ-1 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
HB-100 100 nmole HPLC 2 ODs
HB-200 200 nmole HPLC 5 ODs

5' HEX-3'Eclipse (mass QC) Synthesis Scale Purification Method Yield(10-60base)
HE-100 100 nmole HPLC 2 ODs
HE-200 200 nmole HPLC 5 ODs

5'TAMRA NHS Ester-3'BHQ-2 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
TB-100 100 nmole HPLC 2 ODs
TB-200 200 nmole HPLC 5 ODs

5'ROX NHS Ester-3'BHQ-2 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
RB-100 100 nmole HPLC 2 ODs
RB-200 200 nmole HPLC 5 ODs

5'CY3 NHS Ester-3'BHQ-2 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY3-100 100 nmole HPLC 2 ODs
CY3-200 200 nmole HPLC 5 ODs

5'CY5 NHS Ester-3'BHQ-2 (mass QC) Synthesis Scale Purification Method Yield(10-60base)
CY5-100 100 nmole HPLC 2 ODs
CY5-200 200 nmole HPLC 5 ODs

 Dark Quenchers
3' Dabcyl Synthesis Scale Purification Method Yield(10-60base)
DAB5-100 100 nmole HPLC 2 ODs
DAB5-200 200 nmole HPLC 5 ODs
DAB5-400 400 nmole HPLC 10 ODs
DAB5-1000 1 µmole HPLC 20 ODs

5' Dabcyl NHS Ester Synthesis Scale Purification Method Yield(10-60base)
DAB5-100 100 nmole HPLC 2 ODs
DAB3-200 200 nmole HPLC 5 ODs
DAB3-400 400 nmole HPLC 10 ODs
DAB3-1000 1 µmole HPLC 20 ODs

3' BHQ-1 Synthesis Scale Purification Method Yield(10-60base)
BHQ1-100 100 nmole HPLC 2 ODs
BHQ1-200 200 nmole HPLC 5 ODs
BHQ1-400 400 nmole HPLC 10 ODs
BHQ1-1000 1 µmole HPLC 20 ODs

3' BHQ-2 Synthesis Scale Purification Method Yield(10-60base)
BHQ2-100 100 nmole HPLC 2 ODs
BHQ2-200 200 nmole HPLC 5 ODs
BHQ2-400 400 nmole HPLC 10 ODs
BHQ2-1000 1 µmole HPLC 20 ODs

3' Eclipse Synthesis Scale Purification Method Yield(10-60base)
ECL-100 100 nmole HPLC 2 ODs
ECL-200 200 nmole HPLC 5 ODs
ECL-400 400 nmole HPLC 10 ODs
ECL-1000 1 µmole HPLC 20 ODs

 Dark Quenchers
5'6-FAM-3'Dabcyl Synthesis Scale Purification Method Yield(10-60base)
FD-100 100 nmole HPLC 2 ODs
FD-200 200 nmole HPLC 5 ODs

5'HEX-3'Dabcyl Synthesis Scale Purification Method Yield(10-60base)
HD-100 100 nmole HPLC 2 ODs
HD-200 200 nmole HPLC 5 ODs

5'TAMRA NHS Ester-3'Dabcyl Synthesis Scale Purification Method Yield(10-60base)
TD-100 100 nmole HPLC 2 ODs
TD-200 200 nmole HPLC 5 ODs


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