| Next Generation Sequencing |
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| Long Sequence Reads/ Shorterst Run Time/ Widest Application |
| • Up to ~ 400bp/reads |
| • Up to ~ 500 Mbp/run |
| • 10hr/ Titanium run |
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| Technology Applications |
DNA Sequencing
> DNA Fragment Library Sequencing
> DNA Mate-pair Library Sequencin |
RNA Sequencing
> Small RNA Sequencing
> Digital Gene Expression-Tag Sequencing
> Transcriptome Sequencing |
Meta-genomics
> DNA Methylation Sequencing
> ChIP-Seq |
Others
> Meta-Genomics DNA Sequencing
> Plasma DNA Sequencing |
| Sample requirement |
| DNA must be double stranded |
| DNA should not be degraded |
| DNA should contain no particulate matter |
| DNA should have an OD 260/280 ratio of above 1.8 |
| DNA sample should have a minimal concentration of 5ng/µl in TE |
| Total DNA required : 500ng |
| cDNA library : Total RNA and mRNA required > 200ng |
| Amplicons : This procedure requires 5-20ng (genomic DNA) or 1-2ng (plasmid DNA or similar) of starting DNA material, in 1µl of molecular biology grade water |
| Amplicons size should be ranged from 200 to 600bp |
How to Order
Download and Email: You may download the Next Generation Sequencing Request Form and submit your order via e-mail to info@mdgsb.com or ict@mdgsb.com.
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